Evaluation of the In Vitro Bioactivities’ Profiles of Brewers’ Spent Grain Protein and Hydrolysates with and without Cellulase Pretreatment

Brewer’s spent grain is the residue left after separation of wort during beer-brewing process. Although this by-product has been reported to have a high protein content, it normally treated as waste. This work aims isolate bioactive hydrolysates from BSG, and then explore their bioactivities. Two groups (A B) BSG were subjected extraction using alkaline method at pH 12, where group A was pre-treated with cellulase, while B not directly extracted. The final yielded 50.18 ± 1.22% recovery BSGP 66.41 0.37% B. RP-HPLC profiles showed that extracted BSGPs are mainly hydrophilic proteins. proteins by two methods hydrolyzed enzymatically Alcalase ?-chymotrypsin. obtained displayed blood pressure regulation activity antioxidant properties, when assayed angiotensin-converting enzyme assay, 2,2-diphenyl-picryl-hydrazyl ferric power assay. It can be concluded possible extract good quality presents potential source for variety might interest food industries.